此原聲由 西浦智仁, Ann Sally 作曲。
作為NDS平臺大人氣的推理解謎游戲雷頓教授三部曲的最后一作《雷頓教授與最后的時間旅行》，本作的主題是穿越時空，雷頓教授一行將在現代世界和未來世界中穿梭，解開各時代的謎題。在未來世界中，青年路克和阿羅瑪都將一一登場。
如果你喜歡Professor Layton and the Last Time Travel的專輯《游戲原聲 - Professor Layton and the Last Time Travel(雷頓教授與最后的時間旅行)》里的歌曲，可以把下面的歌曲連接發給你的朋友:
1.Theme of the Last Time Travel - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381376.html
2.Puzzles 3 - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381377.html
3.London 2 - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381378.html
4.Searching for Clues - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381379.html
5.Tension - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381380.html
6.London 3 - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381381.html
7.Calm Village - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381382.html
8.Casino Number 7 - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381383.html
9.Inside Sadness - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381384.html
10.Suspicion - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381385.html
11.Asian Street - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381386.html
12.Puzzle Battle - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381387.html
13.With Memory - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381388.html
14.Hexagon Tower - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381389.html
15.The Professor's Reasonings - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381390.html
16.Pinch! - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381391.html
17.Laboratory - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381392.html
18.Puzzles 4 - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381393.html
19.Huge Weapon - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381394.html
20.The Professor's Trunk (Picture Book) - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381395.html
21.The Professor's Trunk (Minicar) - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381396.html
22.The Professor's Trunk (Paro) - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381397.html
23.Time Travel (Piano ver.)(Instrumental) - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381398.html
24.London 3 - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381399.html
25.Laboratory - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381400.html
26.Huge Weapon - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381401.html
27.Hexagon Tower - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381402.html
28.The Professor's Trunk (Minicar) - Professor Layton and the Last Time Travel
http://www.guangchangzhuan.cn/ting/381403.html